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Sesión

Vi-P3: Sesión de pósteres III
Lugar: Zona pósteres

Presentación 8

High-Resolution 3D Imaging of the Zebrafish Heart Using Synchrotron Based X-Ray Phase Contrast Imaging

Mariana Lourenço Seabra¹, Senda Jimenez Delgado^2,3,5, Ángel Raya^2,3,4,5, Alessandra Patera⁶, Gabriel Bernardino¹, Bart Bijnens^1,4

1: BCN-MedTech, Department of Engineering, University Pompeu Fabra, Barcelona, Spain; 2: Program for Clinical Translation of Regenerative Medicine in Catalonia (P-[CMRC]), L'Hospitalet de Llobregat, Spain; 3: Center for Networked Biomedical Research on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; 4: Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain; 5: Stem Cell Potency Group, Regenerative Medicine Program, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain; 6: Alba Synchrotron Light Source, Cerdanyola del Vallès, Spain

The zebrafish heart is a valuable cardiovascular model due to its similarity to the human heart. However, conventional approaches for tissue analysis, such as histology, require destructive slicing, which alters tissue integrity and limits 3D insight. To overcome this, we applied synchrotron-based X-ray phase contrast imaging (X-PCI), a non-destructive, 3D, high resolution, and high contrast method with 0.65 μm resolution, to image an intact zebrafish heart. X-PCI revealed key microstructures, highlighting its potential for cardiac morphology studies.

To develop our protocol, an adult zebrafish heart (AB strain) was fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin. The procedures were approved by the CEEA-PRBB. Imaging was performed at the Alba’s Synchrotron FAXTOR beamline, with a 20 keV beam and a PCO-edge 4.2 sCMOS camera with a 10x objective, giving a isotropic voxel size of 0,65μm and a field of view of 1,3x1,3 mm. Each tomogram comprised 2000 projections (230 ms exposure time)
and was reconstructed with Gridrec.

Synchrotron-based X-PCI enabled high-resolution 3D imaging of the zebrafish heart, allowing clear visualization of myocardial microstructure. Individual cardiomyocytes and clusters of cells were identifiable, with elongated cellular profiles in short-axis sections. This study validates X-PCI as a powerful method for visualizing the intact zebrafish heart in high-resolution 3D. By successfully identifying key microstructures, including individual cardiomyocytes and vessels, within their complete spatial context, our findings highlight X-PCI’s potential for quantitative 3D morphological analysis, aiming to advance the understanding of cardiovascular pathophysiology in this animal model.