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Mi-P1: Sesión de pósteres I Lugar: Zona pósteres P1: Sesión de pósteres I | |
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Porcine Intestinal Tissue Modeling Using Organoids on Microfluidic Organ-on-Chip for Translational Research 1: Universidad de Zaragoza, España; 2: Instituto de Investigación Sanitaria Aragón (IIS Aragón) Physiologically relevant in vitro models are essential to investigate intestinal processes such as nutrient absorption, microbiota interactions, and therapeutic responses. Conventional 2D systems fail to reproduce epithelial complexity, while porcine intestinal organoids (PIOs) preserve cellular diversity but lack dynamic stimulation. In this study, PIO-derived monolayers were cultured under static conditions on Transwell inserts and compared to dynamic cultures on a microfluidic organ-on-chip platform over 14 days. Barrier integrity, alkaline phosphatase activity, immunofluorescence, and RT-qPCR analyses were performed. The chip model displayed faster and more stable barrier maturation, reaching in vivo-like permeability and differentiation levels earlier than static cultures. Gene expression confirmed enhanced epithelial differentiation under flow, with increased VIL1, MUC2, and OCLN, and reduced LGR5 expression. These findings demonstrate that dynamic organoid-on-chip cultures achieve superior functional maturation, establishing a more predictive model of intestinal physiology for translational applications in pharmacology, nutrition, and microbiota research. | |