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Conference Agenda

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

Daily Overview

Session

CP20: Zoonoses & One Health 15 min talks
Location: Lecture Theatre 3
Session Chair: Darren Gray, QIMR Berghofer
Session Chair: Jessica Scott, James Cook University

Presentation 2

Unraveling the whole mitochondrial genome of the highly endemic G3 genotype of Echinococcus granulosus from Pakistan: an emerging epidemiological concern

Hira Muqaddas^1,2, Naunain Mehmood³, Hayley Martinez DeCristi², Katherine Muscat², Nichola Eliza Davies Calvani²

1: Department of Zoology, The Women University Multan, Multan, Pakistan; 2: Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, NSW, Australia; 3: Department of Zoology, University of Sargodha, Sargodha, Pakistan

Introduction

Echinococcus granulosus sensu stricto (s.s.) is a zoonotic parasite that infects a wide spectrum of hosts. A substantial public health concern, this cestode causes socioeconomic burdens to resource-limited pastoral communities globally. The G1 and G3 genotypes of E. granulosus s.s. are associated with human and animal infection, with G1 predominating. However, an opposite trend has been observed in Pakistan, where the G3 genotype is more common.

Materials and Methods

In the present study, genomic DNA was extracted from hydatid cysts obtained from cattle in Multan, Pakistan and processed for whole-genome sequencing (Illumina NovaSeq). Complete mitochondrial genomes were assembled and population genetic analysis was performed and compared to those from public databases.

Results

The results indicated that the G3 genotype of E. granulosus s.s. is highly prevalent among the cattle of Pakistan. Using whole mitogenome data (13,610 bp), when compared to the G1 reference sequence, G3 exhibited substantial variation at 48 diagnostic positions, indicating a distinct mitochondrial lineage. Genetic variability indices revealed high haplotype diversity and low nucleotide diversity.

Conclusions

This study reports the first complete mitogenome of the G3 genotype of E. granulosus s.s. from Pakistan, providing a foundation for future genetic analyses from other regions of the world.