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Conference Agenda

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

Daily Overview

Session

CP23.1: Zoonoses & One Health 2 - 10 min talks
Location: Lecture Theatre 3
Session Chair: Catherine Gordon, QIMR Berghofer
Session Chair: Fasil Shiferaw, QIMR Berghofer

Presentation 1

Multi locus sequence typing (MLST) supports zoonotic transmission of Cryptosporidium parvum in Western Australia

Dinamithra Gedara Sugandika Samanmali Bulumulla¹, Una Ryan¹, Amanda Ash¹, Amanda D Barbosa¹, Joshua Aleri²

1: Murdoch University, Western Australia, Australia; 2: The University of Queensland, Queensland, Australia

Cryptosporidium is a major enteric zoonotic protozoan parasite with a wide host range, causing gastrointestinal disease in humans and animals. Livestock, particularly calves, serve as an important zoonotic reservoir for Cryptosporidium parvum, the main zoonotic species. In the present study, 300 cattle faecal samples (200 calves and 100 adult cattle) from Western Australia (WA) were screened for Cryptosporidium by PCR and sequence analysis at the18S rRNA locus. Cryptosporidium parvum positives were analysed using multi locus sequence typing (MLST) of five polymorphic loci including the hypervariable gp60 gene and compared to previously typed human C. parvum samples from WA.

Results revealed that C. parvum was the only species detected in both calves and adult cattle with three C. parvum gp60 subtype families identified; IIaA18G3R1, IIaA19G4R1 and IIaA20G3R1. MLST analysis demonstrated limited genetic diversity among cattle and human C. parvum isolates, with most isolates clustering within a single dominant clade. The intermixing of host-derived isolates supports potential zoonotic transmission of C. parvum.